Description

Diaphonized Frog in a Vial with Glycerin.

You will receive this specimen, or one like it.  (This is for 1 specimen)

Diaphonizing, also known as clearing and staining, is a fascinating scientific technique used to study the internal anatomy of small animals—especially fish, reptiles, amphibians, and even small mammals—without dissection. It renders the soft tissues transparent (“cleared”) while staining the bones and cartilage in vivid contrasting colors, creating a visually striking and educational display.

Here’s how it works, step by step:

1. Fixation:
   The specimen is first preserved in a fixative like formalin to halt decay and stabilize tissues.

2. Staining:
   Specific dyes are used to color the internal structures:

   • Alizarin Red S binds to calcium, staining bones a deep red or magenta.

   • Alcian Blue binds to cartilage, turning it a vivid blue or turquoise.
     These contrasting colors make skeletal details stand out beautifully.

3. Clearing:
   After staining, the soft tissues are treated with digestive enzymes (often trypsin) that gradually dissolve muscle and connective tissue—but leave the skeleton and skin intact.
   Then, the specimen is immersed in glycerin, which makes what remains of the soft tissue optically clear.

4. Preservation:
   The finished diaphonized specimen is stored in glycerin (sometimes with thymol to prevent mold), often in a glass vial or jar, where it can be admired indefinitely.

The result is almost otherworldly—a ghostlike creature whose skeleton glows within a transparent body. Scientists use diaphonization to study skeletal development, deformities, and evolutionary relationships, while collectors and artists appreciate them for their haunting beauty and anatomical precision.

Diaphonized Pinky Mice